JRC GMO-Amplicons | EURL GMFF

Record ID:	OP963968.1
Database:	nt	Go to original record
Description:	Leontopodium calocephalum voucher Q.F. Zhao et al. ZSX1908251 chloroplast, complete genome.
Taxon:	Leontopodium calocephalum
Original size:	151094 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46659	47184	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aataaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacaaatgg agttgattgt cttacattgg tagagaaatc cttctatcga
251   aacttcagaa aagatgaagg ataaacctgt atacataata gagatacaga
301   agaattgata tgaatcgatt ccatatttaa ttgaagaaag aatcgaatat
351   tcattgatca aaccattcac tccataatct gatagatctt ttgaagaact
401   gattaatcgg acgagaataa agatagagtc ccgttctaca tgtcaatacc
451   ggcaacaatg aaatttatag taagaggaaa atccgtcgat ttcaaaaatc
501   atgagggttc aagtccctct atcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details