JRC GMO-Amplicons | EURL GMFF

Record ID:	OP186464.1
Database:	nt	Go to original record
Description:	Nepeta hemsleyana voucher H3050095 chloroplast, complete genome.
Taxon:	Nepeta hemsleyana
Original size:	152177 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46885	47422	+	538 bp

Amplicon:

538 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggataggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tgcgccggta
201   gaggaatctt tccatggaaa ctttagaaag gataaacgca tctattgaat
251   actatatcaa atgattaatg ttggcccgaa tccgtttttt ttaatatgaa
301   aatgaaaaaa tggaaaaatc ggtgtgaatt tatttcacgt tgaagaaaaa
351   atcgaatatt catcaactca ttcactccat agtccgatag atcttttaaa
401   taactgatta atcggacgag aataaagata gagtcccatt ctacatgcta
451   catgtgaata ccggcaacaa tgaaatttat agtaatagga aaatccgtcg
501   actttaaaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	538	519	2.42e-08

Match details