JRC GMO-Amplicons | EURL GMFF

Record ID:	OP169573.1
Database:	nt	Go to original record
Description:	Eremophila capricornica isolate BMR01800 chloroplast, complete genome.
Taxon:	Eremophila capricornica
Original size:	151593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48162	48717	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggataaagg tatatattga atactatctc aaatgattaa tgatggccct
301   aatctgtatc tgtatttttt tatatgaaaa atggaagaat tggtatgaag
351   tgattccaca ttgaagaaag aatcaaatat tccttcatca aatcattcac
401   tccacagtct gatagttctt ttaaagaact gattaatcgg acaagaataa
451   agatagagtc ccattctaca tgtcaatatt ggcaacaatg caatttatag
501   taagaggaaa atccgtcgac tttaaaagtc gtgagggttc aagtccctct
551   atcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details