JRC GMO-Amplicons | EURL GMFF

Record ID:	ON815627.1
Database:	nt	Go to original record
Description:	Phlomoides rotata isolate M106 voucher KUN-EM1072 plastid, complete genome.
Taxon:	Phlomoides rotata
Original size:	151877 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46709	47268	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac gaactaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttgcgttgg tagaggaatc ctttctacgg
251   aaacttcaga aaggataaac gtatctatcg aatactatat caaatgatta
301   atgatggccc gaatccgtat ttttaatatg aaaaatagaa gaattggtgt
351   gaattgattc tatattgaag aaaaaatcga atattcattc atcaaatcat
401   tcactccata gtccgataga tcttttaaag aactgattaa tcggacgaga
451   ataaagatag agtcccattc tacatgtcaa tactggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details