JRC GMO-Amplicons | EURL GMFF

Record ID:	ON815620.1
Database:	nt	Go to original record
Description:	Phlomis herba-venti subsp. pungens isolate M21 voucher MW0723729 plastid, complete genome.
Taxon:	Phlomis herba-venti subsp. pungens
Original size:	151571 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46538	47099	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttt aaaacaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatacttt atcaaatgat
301   taatgatgga ccgaatccgt atttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga agaaaaaatc gaatattcat tcatcaaatc
401   attcactcca tagttcgata gatcttttaa agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aatactggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details