JRC GMO-Amplicons | EURL GMFF

Record ID:	OM804224.1
Database:	nt	Go to original record
Description:	Lycium barbarum clone V12L0031 Ningnongqi No.5 tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, and tRNA-Phe (trnF) gene, complete sequence; chloroplast.
Taxon:	Lycium barbarum
Original size:	1155 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	112	706	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cattggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactcta
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat aaaaaataga
351   agaattggtg tgaatcgatt ctacagaatt ggtgtgaatc gattctacat
401   tgaagaaaga atcgaatatt cattgatcaa atcattcact ccatagtctg
451   atagatcctt tgaagaactg attaatcgga cgagaataaa gatagagtcc
501   cgttctacat gtcaataccg gcaacaatga aatttatagt aagaggaaaa
551   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details