JRC GMO-Amplicons | EURL GMFF

Record ID:	MW392092.1
Database:	nt	Go to original record
Description:	Plumeria filifolia chloroplast, partial genome.
Taxon:	Plumeria filifolia
Original size:	150393 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48055	48618	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttt cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctat tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccgtcgaaaa ttcagaaagg
251   atgaagtata aacgtatata catacgtatt gaatactata tcaaatgatt
301   aatgccaacc cgaatgaatc tgtatttttt ctataaaaac tggaagaatt
351   ggtgtgattc gattctttct tcaatgtgga atccaatatt cattgatcaa
401   atgattcact ccacagtctg tagatctttt caagaactga ttaatcggac
451   gagaataaag atagagtccc gttctacatg tcaatgctgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt aaaaaatcgt gagggttcaa
551   gtccctctat cccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details