JRC GMO-Amplicons | EURL GMFF

Record ID:	OP007396.1
Database:	nt	Go to original record
Description:	Buddleja fallowiana chloroplast, complete genome.
Taxon:	Buddleja fallowiana
Original size:	154130 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48039	48597	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgttgt ctcaaaacaa aggttcagaa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttaact gcgttggtag cggaatcttt cgatcgaaac ttcagaaaga
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   accgaatctg tattttttta tatgaaaaat ggaaaaattg gtgtgaattt
351   attccacatt gaagaaagaa tcaaataaaa tattcattca tcaaatcatt
401   cactccatag tctgatagat cttttaaaga actgattaat cggacgagaa
451   taaagataga gtcccattct acatgtcaat accggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 22 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details