JRC GMO-Amplicons | EURL GMFF

Record ID:	OP007375.1
Database:	nt	Go to original record
Description:	Buddleja asiatica chloroplast, complete genome.
Taxon:	Buddleja asiatica
Original size:	154172 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48090	48647	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcgaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttaactg cgttggtagc ggaatctttc gatcgaaact tcagaaagag
251   tgacggataa acgtatctat tgaatactat atcaaatgat taatgatgga
301   cctaatctgt atttttttat atgaaaaatg gaaaaattgg tgtgaattta
351   ttccacattg aagaaagaat caaataaaat attcattcat caaatcattc
401   actccatagt ctgatagatc ttttaaagaa ctgattaatc ggacgagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details