JRC GMO-Amplicons | EURL GMFF

Record ID:	OP007366.1
Database:	nt	Go to original record
Description:	Buddleja alternifolia chloroplast, complete genome.
Taxon:	Buddleja alternifolia
Original size:	154216 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48123	48696	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgttgt ctcaaaacaa aggttcagaa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttaact gcgttggtag cggaatcttt cgatcgaaac ttcagaaaga
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   accgaatctg tattttttta tatgaaaaat ggaaaaattg gtgtgaattt
351   attccacatt gaagaaagaa tcaaataaaa tattcattca tcaaatcatt
401   cactccatag tctgatagat cttttaaaga actgattaat aaagaactga
451   ttaatcggac gagaataaag atagagtccc attctacatg tcaataccgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details