JRC GMO-Amplicons | EURL GMFF

Record ID:	ON453817.1
Database:	nt	Go to original record
Description:	Campsis grandiflora chloroplast, complete genome.
Taxon:	Campsis grandiflora
Original size:	154282 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47517	48078	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtt ggtagaggaa tctttccatc gaaacttcag
251   aaaggatgaa ggataaacgt atctattgaa tactatatca aatatcaaat
301   gattaatgat ggcccgaatc tgtatctgta tttttaatgg aaaaattggt
351   gtgaattgat tccacattga agaaagaatc gaatattcat tcatcaaatc
401   attcactcca tagtccgata gttcttttaa agaactgatt aatcggacaa
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgactttc aaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details