JRC GMO-Amplicons | EURL GMFF

Record ID:	MW279182.1
Database:	nt	Go to original record
Description:	Amana anhuiensis clone 1 chloroplast, complete genome.
Taxon:	Amana anhuiensis
Original size:	151573 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45512	46078	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttt ttcaaaaaaa aggataggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgat tacgttacat
201   gcgctagtag ccggaatcct tctatcaaaa ttattgaaaa gatacccgcc
251   ctatatatgt agatatacat actcacatag taaacgattg attaatcgca
301   gcccaaatcc attatatatg aaaaatttaa gaatcattgt gaatctattc
351   cattcgatat tccaatcgaa gttggaggaa gaatcgaata ttaaatgatc
401   aaattattca ttccaaagtc tgatagatct tttgaaaaac cgattattcg
451   aacgagaata aagagagagt cccattctac gtgtcaatac cgacaacaat
501   gaaatttata gtaaaaggaa aatccgtcga ctttaaaagt cgtgagggtt
551   caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details