JRC GMO-Amplicons | EURL GMFF

Record ID:	ON408227.1
Database:	nt	Go to original record
Description:	Tetraena mongolica plastid, complete genome.
Taxon:	Tetraena mongolica
Original size:	106177 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47077	47612	+	536 bp

Amplicon:

536 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttctg aaaaaaaaaa ataattcaat
151   caaaagaaaa agaatcaaaa aaggataggt gcagagactc aatggaagct
201   gttctaaaaa atggagttga ctgctgcatt atgttagtca agtcaaggaa
251   tccttgcata aaaactttca aaaagatgaa ggataaactt tctttctata
301   aaactctaaa aagaaaaata ctttttcaat caattggaag ttgaataaag
351   aatcaaatat aatttgatca aatcatttac tccaaagtct gatatatctt
401   tttcaaaatg gattagtcag atgagaataa agatagagtc ccattctata
451   tgtcagtatt gacaacaatg aaatttatag tgagaggaaa atccgtcgat
501   tttataaatc gtgagggttc aagtccctct atcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	536	517	2.41e-08

Match details