JRC GMO-Amplicons | EURL GMFF

Record ID:	ON858926.1
Database:	nt	Go to original record
Description:	Isodon coetsa var. coetsa chloroplast, complete genome.
Taxon:	Isodon coetsa var. coetsa
Original size:	152658 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47109	47641	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa ggataggtgc
151   agagactcaa tggaagctgt tctaacaaat gggagttgac tgcgctggta
201   gaggaatctt tccatggaaa ctttagaaag gatgaaggat aaacgcatca
251   tactatatca aaatcaaatg gttaatgatg gcccgaatct tttaatataa
301   aaaaatggaa aaattggtgt gaattgattc tacgttgaag aaaaaatcga
351   atattcatca actcattcgc tccatagtcc gatagatctt ttaaagaact
401   gattaatcgg acgagaataa agatagagtc ccattctaca tgctacatgt
451   caatatcggc aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
501   aaaaatcgtg agggttcaag tccctctatc ccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details