JRC GMO-Amplicons | EURL GMFF

Record ID:	ON858892.1
Database:	nt	Go to original record
Description:	Isodon serra chloroplast, complete genome.
Taxon:	Isodon serra
Original size:	152551 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46983	47536	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa ggttcaaaaa
151   acgaaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tgggagttga ctgcgctggt agaggaatct ttccatggaa actttagaaa
251   ggatgaagga taaacgcatc atactatatc aaaatcaaat ggttaatgat
301   ggcccgaatc ttttaatata aaaaaatgga aaaattggtg tgaattgatt
351   ctacgttgaa gaaaaaatcg aatattcatc aactcattcg ctccatagtc
401   cgatagatct tttaaagaac tgattaatcg gacgagaata aagatagagt
451   cccattctac atgctacatg tcaatatcgg caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

View other 15 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details