JRC GMO-Amplicons | EURL GMFF

Record ID:	OX335502.1
Database:	nt	Go to original record
Description:	Musa cultivar 'New-Mu-Nang' complete chloroplast sequence
Taxon:	Musa ABB Group
Original size:	170318 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49817	50425	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttattttga gaaaacaaag gtttataaaa
151   ctagaattta aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatggagttg actacgtttt gttggtagct ggaatccgtc tatcaaaatt
251   acagaaaaga tggtcctata tacctaatac gtatgtatac atactgccat
301   atcaaacgat taatcatgac ccgaatctat tatattataa ttatatataa
351   ttataatatg aaaaattata atatgaaaaa ttcagagtta ttatgaatcc
401   atttcaatgg aagttgaagg aagaatcgaa tattcaatta tgaaatcatt
451   cattccagag tttgatagat cttttgaaaa actgattaat cggacgagaa
501   taaagagaga gtcccattct acatgtcaat accgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
601   tctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details