JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641364.1
Database:	nt	Go to original record
Description:	Salvia officinalis isolate BPTPS165 voucher LISE:96476 chloroplast, complete genome.
Taxon:	Salvia officinalis
Original size:	151164 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46213	46773	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgcgc cggtagagga atctttccat ggaaacttta
251   gaaaggatga aggataaacg cacctattga atactatatc aaatgattaa
301   tgttgacccg aatctgtttt tttaatatga aaatggaaaa ataggtgtga
351   atttatttca cgttgaagaa taaatcgaat attcctcaac tcattcactc
401   catagtccga tagatctttt aaagaactta ttaatcggac gagaataaag
451   atagagtccc attctacatg ctacatgtca ataccggcaa caatgaaatt
501   tatggtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details