JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684562.1
Database:	nt	Go to original record
Description:	Clinopodium menthifolium subsp. ascendens isolate BPTPS184 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Clinopodium menthifolium subsp. ascendens
Original size:	575 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	574	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttcaaa
151   aaacgaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgtcggta gaggaatctt tccatggaaa ctttctaaag
251   gatgaaagat aaacgcatct attgaatact atatcaaatg attaatgttg
301   gcccgaatct atttttttaa tatgaaaatg aaaaaatgga aaaatcggtg
351   tgaatttctt tcacgttgaa gaaaaaatag aatattcatc aactcattca
401   ctccacagtc cgatagatct tttaaagaac ttattaatcg gacgagaata
451   aagatagagt cccattctac atgctacata tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details