JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684558.1
Database:	nt	Go to original record
Description:	Mentha aquatica isolate BPTPS173 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Mentha aquatica
Original size:	581 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	580	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtcggt agaggaatct ttccatggaa actttatttt
251   ataaaggatg aaagataaac gcatctattg aatactatat caaatgatta
301   atgttggccc gaatctattt ttttaatatg aaaatgaaaa aatggaaaaa
351   tcggtgtgaa tttttttcac gttgaagaaa aaatagaata ttcatcaact
401   cattcactcc gtagtccgat agatctttta aagaacttat taatcggacg
451   agaataaaga tagagtccca ttctacatgc tacatatcaa taccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details