JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684549.1
Database:	nt	Go to original record
Description:	Satureja montana isolate BPTPS148 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Satureja montana
Original size:	574 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	573	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttcaaa
151   aaacgaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgtcggtag aggaatcttt ccatggaaac tttataaagg
251   atgaaagata aacgcatcta ttgaatacta tatcaaatga ttaatgttgg
301   cccgaatcta tttttttaat atgaaaatga aaaaatggaa aaatcggtgt
351   gaatttattt cacgttgaag aaaaaataga atattcatca actcattcac
401   tccatagtcc gatagatctt ttaaagaact tattaatcgg acgagaataa
451   agatagagtc ccattctaca tgctacatat caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details