JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684533.1
Database:	nt	Go to original record
Description:	Cistus ladanifer isolate BPTPS031 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Cistus ladanifer
Original size:	599 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	598	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agcaggtcaa aaagcaataa
151   aaaataggat aggtgcagag actcaatgga agttattcaa acaaatggga
201   ttgaatggct ttgcgttggt aaaagaatac ttctatcaaa actcaagcaa
251   ggataaacat ataaaataaa ctatttcaag aaaaaaatag gaatggggta
301   cctattcttt tgatttactt tataagttgt agcaaaaaga attttataca
351   aaatcaaaag aatcattctg aatccattgc aagttaatga atagaatatt
401   cattaataaa aaagaaaacc attcactcca taatctgata aatcttttga
451   aaaacagatt aatcggacga gaataaagat agagtcccgt tctacatgtc
501   aatatcaata ccgacaacaa tgaaagttat agtaagagga aaatccgtcg
551   actttaaaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details