JRC GMO-Amplicons | EURL GMFF

Record ID:	MT526782.1
Database:	nt	Go to original record
Description:	Amaranthus standleyanus voucher 7433 chloroplast, complete genome.
Taxon:	Amaranthus standleyanus
Original size:	150569 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47368	47982	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaaatacg
151   gattcagaaa gcaagaataa aaaaaaggat aggtgcagag actcaaagga
201   agctgttcta acaaatggag ttgattgtgc tgggttgttc gaagaattct
251   tccgtcgaaa ttcctgatta atggcacttc gaatctattc tgtatttcta
301   tttttagaat ttttagatat ttctgatttc tagataatat agaatatgaa
351   attttctaga aataaaaatg aatatgaaaa aatggaagaa tttttttgac
401   tctatttcaa gttgaaaaaa gaatcaaata ttcatcaaat cattcactcc
451   atagtctgat agatctttct ttgaaagaac cggttaatcg gttactcgga
501   cgagaataaa gatagagtcc cattatacat gtcaatactg acaacaatga
551   aatttatagt acgaggaaaa tccgtcgact ttaaaaatcg tgagggttca
601   agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details