JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ748477.1
Database:	nt	Go to original record
Description:	Persicaria perfoliata chloroplast, complete genome.
Taxon:	Persicaria perfoliata
Original size:	160680 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48644	49244	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaagaa
101   atgggcaatc ctgagccaac tcctgctttc caaaaggaaa gaaaaagagg
151   ataggtgcag agactcaatg gaagctgttc taacaaaggg aattgactgt
201   cttgcgttgt aatatgttat aagaattttt cgatctaaac tccaaaagtg
251   atgaagaaga aacctatagg tatacgtact taaatagaaa gactatacta
301   tgatataata aataaaaaat gcaaaaatat tgataacgac ccgaattttt
351   attttatgaa tatgaaaaaa atggaagaat ttttgtgaat cgattccaag
401   ttgaagaaag aatcgaatat tcgtttatta aattaaagca tttactccac
451   ggtttggtag atcttttgaa gaactgaccc atcggatgag aatgaagata
501   gagtcccatt ctacgtgtca atcccgacac caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc cctctatccc
601   c

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details