JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ953910.1
Database:	nt	Go to original record
Description:	Periploca sepium voucher T. Livshultz s.n. 28.VII.2009 (PH) plastid, partial genome.
Taxon:	Periploca sepium
Original size:	143188 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47087	47645	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaaaaaa
101   aagggcaatc ctgagccaaa tcctgtttta cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagatgt tctaacaaat
201   ggaggtggcc acgttggtag agaaatcctt ccatcaaaat taagaaagga
251   tgaaggataa acacatatac atacgttttg aatactatat caaatgatta
301   tgccgacccg aatgaatccg tttttttttt ttctataaaa aaggaagaat
351   tggtgtgagt ctattctttc ttcaatgtgg aatcgaatat tcattgatca
401   aatgattcac tccatagtct gtagatccga acggattaat cggacgagaa
451   taaagataga gtcccgttct acatgccaac gctggcaaca atgaaatttt
501   gagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details