JRC GMO-Amplicons | EURL GMFF

Record ID:	MW559062.1
Database:	nt	Go to original record
Description:	Averrhoa dolichocarpa voucher EAW8831 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Averrhoa dolichocarpa
Original size:	978 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	568	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acgtactaag tgagaacttt caaattcagc gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttttttccg aaaacaaaca aagttttaga
151   aagcgagact aaaaaaggat aggtgcagag actcaacgga agctattcta
201   aaatttacaa atagagttga ctgcgttgcg ttagtaaggg aatccttccc
251   tcaaaattcc agaaaggata aacctatata catacgtata cgtactgaaa
301   tactatctca aatgattaat gatgacctga atctgtatta tttatacaaa
351   taatgaaaga tttcttgtga gtcgattcca agttgaagaa agaatcgaat
401   gataattgat caaattattc attccagttc ggatagatct tttgaaaacc
451   tgatagaata aagatagagt cccattctac atgtcaatat tgacaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttagaaat cgtgagggtt
551   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details