JRC GMO-Amplicons | EURL GMFF

Record ID:	MW689264.1
Database:	nt	Go to original record
Description:	Vitex negundo chloroplast, complete genome.
Taxon:	Vitex negundo
Original size:	154471 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48141	48699	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacaaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtat aggaattttt ccgtgaaaac ttcagaaagg
251   atgaaggata aacgtctcta ttaaatacta tatcaaatga ttaatgatgg
301   cccgaatctg tatctgtatt tttaatatga aaaatggaag aattggtgtg
351   aattgattcc acattgaaga aaaaaccgaa tattcattca tcaaatcatt
401   cactccatag tccgatagtt cttttaaaga actgattaat cggacgagaa
451   taaagataga gtcccattct acatgccaat accggcaaaa atgaaattta
501   tagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details