JRC GMO-Amplicons | EURL GMFF

Record ID:	MW181770.1
Database:	nt	Go to original record
Description:	Clerodendrum japonicum chloroplast, complete genome.
Taxon:	Clerodendrum japonicum
Original size:	152217 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47168	47726	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttgctaag tgatgacttt caaattcaga gaaaccccgg aactaattaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   agcgaaaaaa aaaaaggata ggtccagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgct ggtagaggaa tctttccatg gaaacttcag
251   aaaggatgaa ggataaatgg atctattgaa tactatatcg aatgattaat
301   gatggcccca atctgtattt tttaatatga aaaatggaag aattggtgtg
351   aattgattcc acattgaaga aaaaatcgaa tattcattca tcaaatcatt
401   cactccacag tccgatagat cttttcaaga tctgattaat cggccgagaa
451   taaagataga gtcccgttct acatgtcaat accggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details