JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ000047.1
Database:	nt	Go to original record
Description:	Echinochloa crus-galli var. crus-galli chloroplast, complete genome.
Taxon:	Echinochloa crus-galli var. crus-galli
Original size:	139800 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48346	48966	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaagaaaagg ataggtgcag agactcaatg gaagttgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tagagaaaga agggctttat acatataata cacacgtata gatactgaca
301   tagcaaacga ttaatcacgg aacccatatc ataatatagg ttctttattt
351   gatttttttt agaatgaaat ttggaatgat tatgaaatag aaaattctga
401   atttttttag aattattgtg aatccattcc aatcgaatat tgagtaatca
451   aatccttcaa ttcattgttt tcgagatctt tacttttaaa aagtggatta
501   atcgaacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details