JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729793.1
Database:	nt	Go to original record
Description:	Reaumuria soongarica clone 3 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria songarica
Original size:	1032 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	591	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   aaaacaaatt cttaaagtga gaagaaataa tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgtgttgtta taagaattct
251   tccatgaatt cttccatcaa aacttaaaaa aagatcaaga aaaaccctat
301   atttagataa atacgtactg aaatactcta aaaaagaaaa aaacgtctta
351   atgataatga cggccaaaat atgtcttttt tatatgaaaa atggaagaat
401   attcatttat caaagtattc gctccatggt ctgatagatt tttttttgac
451   gaactgctca atcggacgag aataaagata gagtcccatt ctacatgtca
501   atactgacaa caatgaaatt tatagtatga ggaaaatccg tcgactttag
551   aaatcgtgag ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details