JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729792.1
Database:	nt	Go to original record
Description:	Reaumuria soongarica clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria songarica
Original size:	1037 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	595	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   aaaacaaatt cttaaagtga gaagaaataa tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgtgttgtta taagaattct
251   tccatgaatt cttccatcaa aacttaaaaa aagatcaaga aaaaccctat
301   atttagataa atacgtactg aaatactcta aaaaagaaaa aaaaaacgtc
351   ttaatgataa tgacggccaa aatatgtctt ttttatatga aaaatggaag
401   aatattcatt tatcaaagta ttcgctccat ggtctgatag attttttttt
451   tgacgaactg ctcaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaatactg acaacaatga aatttatagt atgaggaaaa tccgtcgact
551   ttagaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details