JRC GMO-Amplicons | EURL GMFF

Record ID:	MW288928.1
Database:	nt	Go to original record
Description:	Cucumis melo subsp. agrestis chloroplast, complete genome.
Taxon:	Cucumis melo subsp. agrestis
Original size:	156017 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49407	50005	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttttttccg aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgacgac gttgcgttag
201   taaaggaatg aatccttcca tcgaaactcc agaaacgatg aaagataaac
251   gtattacgta ctgaaatact atattgatta atgacaactc gaatctctat
301   tttttatatt tctataattt tttatattta tatatatata taaaattata
351   tataaaaatg aacgaattgt tatgaatcga ttccaagatc tacaagttga
401   aaaaataatc gaatattcat tgatcaaatc atttactcca tcatagtctg
451   atagatcttt tgaagaactg attaatcaga tcgaatagaa taaagataga
501   gtcccattct acatgtcaat aacgacaaaa atgaaattta tagtaagagg
551   aaaatccgtc gacttttaaa atcgtgaggg ttcaagtccc tctatcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details