JRC GMO-Amplicons | EURL GMFF

Record ID:	MW246825.1
Database:	nt	Go to original record
Description:	Anisodus tanguticus chloroplast, complete genome.
Taxon:	Anisodus tanguticus
Original size:	152714 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46575	47173	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaatt aagggatttc
151   tgaaagcgag aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaatgaat ggagttgact gcgttggtag aggaatcctt ctattcaatt
251   ttaatttaaa ttccataaag aatgagggat gaacctatat acgatacgta
301   cgtatacgta ctgaaatatc aaagattcta ttaatgccgc cctaaatata
351   tattttttac ataaaaaaag gaataatttt tgtgaatcta ttccaagttg
401   aaggaagaat agaaaattca gtgatcaaac cattcactcc atagtctgat
451   agatcttttg acgaactgat taatcggacg agaataaaga tagagtcccg
501   ttctacatgt caatatcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttataa atcgtgaggg ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details