JRC GMO-Amplicons | EURL GMFF

Record ID:	MW186709.1
Database:	nt	Go to original record
Description:	Orinus intermedius chloroplast, complete genome.
Taxon:	Orinus intermedius
Original size:	134283 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46375	46987	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acttgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtgtaatta cgttgtgttg gtagtgaaac tccctctaaa
251   ttacaaagaa gggctttata catctaatac acacgtatag atactgacat
301   agcaaacgat taatcacaga acccatacca taatataggt tctttattta
351   ttttttagaa tgaaattagg aatgattatg aaatagaaaa ttctgaattt
401   tttttagaat tattgtgaat acattccact cgaatattga gtaatcaaat
451   ccttcaattc attgtttttg agatatttta aaaatagaat taatcggacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details