JRC GMO-Amplicons | EURL GMFF

Record ID:	MW331536.1
Database:	nt	Go to original record
Description:	Sinopodophyllum hexandrum chloroplast, complete genome.
Taxon:	Sinopodophyllum hexandrum
Original size:	157255 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49348	49886	+	539 bp

Amplicon:

539 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactgag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcgg aaaagggata ggtgcagaga
151   ctcgatggaa gctgttctaa cgaatgaagt tggtcgaaaa aaatctttct
201   attaaaactc cagaaaggat gaacctatat atgatatata ggtatacgta
251   ctgaaataaa atagaaaaga ttaatgaaat acgaatcctt tatttaactt
301   atacaaaaat tgaaaaattc ttctgaatcg attccatgtg gaagtaagga
351   tcgaatattc actgatcaaa tcagccactt tagagtatag tatgatagat
401   tttgtgaaga actaactaat tggacgagaa taaagataga gtcccattct
451   acatgtcaat accgacaaca atgaaattta tagtaagagg aaaatccgtc
501   gactttagca atcgtgaggg ttcaagtccc tctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	539	520	2.42e-08

Match details