JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ779005.1
Database:	nt	Go to original record
Description:	Plantago asiatica chloroplast, complete genome.
Taxon:	Plantago asiatica
Original size:	165045 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45875	46456	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactagg tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   aaatgggcaa tcctgagcca aatcctgtct tctcaaaata aaggttcata
151   aagcgaaaag gggataggtg cagagactcg atggaagctg ttctaaaaca
201   aatggagttg actgcgttgg tagatgaatc ttttcatcga aacttcagaa
251   aagatgaagg ataaacggat ctattgaata ctaaaatatc taaaaaaaag
301   gaatgacggc ccgagtctgc atctgtattt ttttagatga aaaatagaag
351   aattggtggg aattgattcc acattcaaga aagaatcgaa tattcattca
401   tcaaatcacc ccactccgta gtctgatagt tttagtcttt ttttaaagaa
451   ctgattaatt aattggacga gaataaagat agagtcccgt tctacatgtc
501   aataccgaca gcaatgaaat ttatagtaat aggaaaatcc gtcgactttt
551   aaaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details