JRC GMO-Amplicons | EURL GMFF

Record ID:	MW309811.1
Database:	nt	Go to original record
Description:	Torenia fordii chloroplast, complete genome.
Taxon:	Torenia fordii
Original size:	154007 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48540	49108	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagag
151   aaaaaaaagg ataggtgcag agactcaatg gaagctgttc taacaaatca
201   aatggagttg actgcgttgg tagatgaatc ttttcatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatataaaa atcaaatgat
301   taatgatggc ccgaatctgt atttgtactt ttttatatga aaaatggaat
351   aattgctgtg aattgattcc acattgaaga aagaatcgaa tattaattca
401   tcaaaccatt cactccatag tctgatagtt cttttaaaga actgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat accggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details