JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ242235.1
Database:	nt	Go to original record
Description:	Scoparia dulcis chloroplast, complete genome.
Taxon:	Scoparia dulcis
Original size:	153701 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47643	48216	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ttcaaaacaa aggttccgaa
151   agtgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aacggagttg actgcgttgg taggggaatc tttccaccaa aacatcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa atcaaatgat
301   taatgatggc ccgaatctct atctctattt ttttctatga aaaacagaag
351   agaagaattg gtgtgaattg attccacatt gaagaaagaa tcgaatattc
401   attcatcacc tcattcactc catagtctga taattctttt aaagaattga
451   ttaatcggac gagaataaag atagagtccc attctacatg tcaataccgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details