JRC GMO-Amplicons | EURL GMFF

Record ID:	MW752129.1
Database:	nt	Go to original record
Description:	Acanthus ilicifolius chloroplast, complete genome.
Taxon:	Acanthus ilicifolius
Original size:	150758 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47141	47675	+	535 bp

Amplicon:

535 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aacgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaataaa
201   tggagttgac tgcaggaatc ttttcatcga aacttcaaag gatagtatct
251   attgaatact atatcaaatg attagataac ccgtatttgt actttttttt
301   tatatgaaaa tggaagaatt ggtgtgaatt gattccacat tgaacaaaga
351   atggaatatt cattcatcaa atcattcact ccatagtccg atagttcttt
401   taaagaactc atcaatcgga cgagaataaa gatagagtcc tattctacat
451   gccaagaccg gcaacaatga aatttatagt aagaggaaaa tccgtcgact
501   ttaaaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	535	516	2.41e-08

Match details