JRC GMO-Amplicons | EURL GMFF

Record ID:	MT513319.1
Database:	nt	Go to original record
Description:	Tetrastigma loheri isolate 3932 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tetrastigma loheri
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	581	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaaccagaa aacgctaata
151   aaaaaaggat aggtgcagag actcaatgga agctgttcta acaaaaacaa
201   atggagttga ctatgttacg atgttaaacg aatccttcca tcaaaactcc
251   agaaccagaa aggatgaagg ataaacctat atacataggt actgaaatat
301   tatatcaaat gattaatgat ggctcgaatc tctatttttt tatatgaaaa
351   atgaaagaat tgttgtaaat cgattccaag ttgaagaaag aaaagaatcg
401   aatattcatt gatcaaatca ttcactccat agtccgatag atcatttgaa
451   gaacttatta atcggacgag aataaagaga gagtcccatt ctacatgtca
501   atatcgacag caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgtgag ggttcaagtc cctctatccc c

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details