JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ145043.1
Database:	nt	Go to original record
Description:	Stipa capillata isolate KRA0496240 haplotype B chloroplast, complete genome.
Taxon:	Stipa capillata
Original size:	137823 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	884	1481	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggttctcgaa
151   ctagaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatcgagt
201   taattacgtt gtgttggtag tggaactcct tctaaattag agagagaggg
251   gctttataca tctaataaac acgtatagat actgacatag caaacgatta
301   atcacagaac ccatatcata attaggttct ttattctttt ttagaataaa
351   attaggaatg attatgaaat aaaaaattct gaattttttt cgaattattg
401   tgaattcatt ccaatcgaat attgagtaat caaatccttc aattcatagt
451   tttcgagatc ttttaaaaag tggattaatc ggacgaggac aaagagagag
501   tcccattcta catgtcaata ctggcaacaa tgaaatttct agtaaaagga
551   aaatccgtcg actttataag tcgtgagggt tcaagtccct ctatcccc

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details