JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610097.1
Database:	nt	Go to original record
Description:	Hyparrhenia rufa isolate TK108 chloroplast, partial genome.
Taxon:	Hyparrhenia rufa
Original size:	138643 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47185	47714	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttga gaatgaaatt tggaaggatt atgaagaggt
301   atagaaaatt cataattttt ttagaattat tgtgaatcta ttccaatcga
351   atattgagta atcaaatcct tcaattcatt gttttcgaga tcttttaaaa
401   agtggattaa tcggacgagg ataaagagag agtcccattc tacatgtcaa
451   tactgacaac aatgaaattt ctagtaaaag gaaaatccgt cgactttata
501   agtcgtgagg gttcaagtcc ctctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details