JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414320.1
Database:	nt	Go to original record
Description:	Salvia splendens isolate 511190055 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia splendens
Original size:	905 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	557	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaacaaa acaaaggttc
151   aaaaaacgaa aaacaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ccggtagagg aatttttcca cggaaacttt
251   agaaaggata aacgcaccta ttgaatacta tatcaaatga ttaataatga
301   ctaatgttga ccccaatctg ttttttaata tgaaaatgga aaaatcgatg
351   tgaatttttt tcccgttgaa gaataaatcg aatattcatc aactcattca
401   ctccatagtt cgatagatct tttaaagaac ttattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaatac cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details