Match details

Record ID: JQ958269.1
Database: nt Go to original record
Description: Globulostylis sp. BV-2013a voucher Dessein et al 1448 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon: Globulostylis leniochlamys
Original size: 1701 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 816 1371 + 556 bp

Amplicon: 556 bp
1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    cccactaagt gataactttc aaattcagag aaaccctgga attaataaaa
101   aggggcaatc ctgagccaaa tcctgttttc cgaaaccaaa gcaaaggttc
151   agaaagtgaa aaagggatag gtgcagagac tcgatggaag ttgttctaac
201   aaatggagtt ggctgcgtta gtaaagaaat ctttccatcg aaaattccga
251   aaggataaaa cgtatacgta ttgaatacta tatcaaatga ttaatgacga
301   ctcgactgaa tccgtatttt ttatatgaaa acggaagaat tggtgtgaat
351   agattccaca ttgaagaaag aatcgaatat tcattgatta aatgattcac
401   tccatagtct gatagatctt ttcaagaatt gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc 

View other 2 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.50e-08
primerB 100.0 20 bp 0 0 556 537 2.50e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.