JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ958215.1
Database:	nt	Go to original record
Description:	Psydrax livida voucher Lemaire & Verstraete 14 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Psydrax livida
Original size:	1637 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	801	1342	+	542 bp

Amplicon:

542 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tgacgactcg
301   actgaatctg tattttttat atgaaaatgg aagaatagat tccacattga
351   agaaagaatc gaatattcat tgattaaatg attcactcca tagtctgata
401   gatcttttca agaattgatt aatcggacga gaataaagat agagtcccgt
451   tctacatgtc aatgtcggca acaatgaaat ttatagtaag aggaaaatcc
501   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	542	523	2.44e-08

Match details