JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047767.1
Database:	nt	Go to original record
Description:	Cyanostegia lanceolata voucher Streiber 57 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cyanostegia lanceolata
Original size:	1518 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	620	1190	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aattaataaa aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa
151   aggtttaaaa agcgaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaacctt tccatggaaa
251   cttcagaaag gatgaaggat aaacatatct attgaatact atatcaaatg
301   attaatgatg gtccgagtct gtatttgtat ttttgaatat aaaaaatgga
351   agaattggtg tgaattgatt ccacattgaa gaaaaaatcg aatattcatt
401   cagcaaatga ttcactccat agtccgatag atcttttaaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca ctaccggcaa
501   caatgaaatt tataataaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details