JRC GMO-Amplicons | EURL GMFF

Record ID:	MW829604.1
Database:	nt	Go to original record
Description:	Ocimum tenuiflorum voucher MH178054 chloroplast, complete genome.
Taxon:	Ocimum tenuiflorum
Original size:	151722 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46449	46992	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatgggagtt gactgtgctg gtagaggaat ctttccatgg aaactttaga
251   aaggatgaag gataaacgca tctattgaat actatatcaa atggttaatg
301   atggcccaaa tcttttaata tgaaaaaatg gaaaaattgg tgtgaattga
351   ttctaggttg aagaaaaaat aaaatattca tcaactcatt cgctccatag
401   tccgatagat aaagaactga ttaatcggac gagaataaag atagagtccc
451   attctacatg tcaatatcgg caacaatgaa atttatagta agaggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details