JRC GMO-Amplicons | EURL GMFF

Record ID:	MF967578.1
Database:	nt	Go to original record
Description:	Ajuga decumbens plastid, complete genome.
Taxon:	Ajuga decumbens
Original size:	150377 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46037	46593	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgatataact ttcaaattca gagaaaccca ggaactaata
101   aaaatgggca atcctgagcc aaatcctgtt ttctcaaaac aaaggttcaa
151   aaaacgaaaa aagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttc actgcgctgg tagaggaatc tttccatgga aacttcagaa
251   aggatgaagg ataaatggat ctattgaata ctatatcgaa tgattcacga
301   tggcccgaat ctgtattttt taatatgaaa aatggaagaa ttggtgtgaa
351   ttgattccac attgaagaaa aaatcgaata ttcattcatc aaatcattca
401   ctccatagtc cgatagatct tttcaagaac tgattaatcg gacgagaata
451   aagatagagt cccgttctac atgtcaatac cggcaacaat gaaatttata
501   gtaataggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details