JRC GMO-Amplicons | EURL GMFF

Record ID:	MF967577.1
Database:	nt	Go to original record
Description:	Mentha canadensis plastid, complete genome.
Taxon:	Mentha canadensis
Original size:	152050 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46886	47457	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgtcg gtagaggaat ctttccatgg aaactttatt
251   ttataaagga tgaaagataa acgcatctat tgaatactat atcaaatgat
301   taatgttggc ccgaatctat ttttttaata tgaaaatgaa aaaatggaaa
351   aatcggtgtg aatttttttc acgttgaaga aaaaatagaa tattcatcaa
401   ctcattcact ccgtagtccg atagatcttt taaagaactt attaatcgga
451   cgagaataaa gatagagtcc cattctacat gctacatatc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details