JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ208887.1
Database:	nt	Go to original record
Description:	Caesalpinia hintonii isolate Inf10 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Erythrostemon hintonii
Original size:	937 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	592	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg acttattgga ttgagccttg gtatggaacc
51    ttactaagtg agaactttca aattcagaga aaccctggaa ttaacaatgg
101   gcaatcctga gccaaatcct gttttccgaa aaccaataag agttcagaaa
151   gggagaataa aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgacatt tcttttcgtt agtaaaggaa tccttccatc
251   taaactccag aaaagaaagg atcaaggatg aacatatata tgtatactga
301   aatattattt caattgatta gaccagacag accaaaaatc tctatttttg
351   aatatttata tgacaaatga aagatgtgaa tagattccaa gttgaagaaa
401   gaatcaaata tttattgatc aaatcattca ttccatgata gtatgataaa
451   tcttttgaag aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aatgaaattt agagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details