JRC GMO-Amplicons | EURL GMFF

Record ID:	MW017634.1
Database:	nt	Go to original record
Description:	Fagopyrum leptopodum chloroplast, complete genome.
Taxon:	Fagopyrum leptopodum
Original size:	159337 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47864	48421	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   cggataatcc tgagccaact cctgctttcc aaaagaaaga ataaatacag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaagc ggagttgact
201   gtattgcgtt gttataagaa ttcttccgtc aaaactcaaa aaaggatgaa
251   gaataaacct atatgcatac gtacgtaaat actaaaaaaa aaaagaataa
301   tattgatgac aacccggata tttattttat gacaaagaag atatgaatca
351   atttcaaatt gaagaaaaaa ttaaatattc atttattatt attaaaagct
401   actcccaagt ctgatggatc ttttaaagaa ccgatccatc ggatgagaat
451   gaagatagag tcccattcta cgtgtcaatc ctgacaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttataaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details